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SRX4948869: PacBio DNA-seq of Tricholomella constricta: mycelium
1 PACBIO_SMRT (PacBio RS II) run: 163,482 spots, 3.6G bases, 11.6Gb downloads

Design: The PacBio libraries were produced using the SMRTbell Template Prep Kit 1.0 according to manufacturer's instructions. 10 g of genomic DNA per library were sheared into 20 kb fragments using the Megaruptor system (Diagenode), followed by an exo VII treatment, DNA damage repair and end-repair before ligation of hair-pin adaptors to generate a SMRTbell library for circular consensus sequencing. The library was then subjected to exo treatment and PB AMPure bead wash procedures and was size selected with the BluePippin system with a cut-off value of 9000 bp.
Submitted by: Lund University
Study: Tricholomella constricta strain:CBS 661.87 Genome sequencing and assembly
show Abstracthide Abstract
The aim of the study was to sequence the genomes of selected litter decomposers from Agaricales, representing the Agaricoid, Marasmioid and Tricholomatoid clades. The comparison of those genomes with other published genomes of mushroom forming fungi aims to: a) to reveal the strategies involved in the decomposition of organic matter and particularly of the plant cell wall, b) to understand the differences in the strategies used between litter and wood decomposers, c) shed light into the evolutionary processes that have shaped those strategies as seen in extant members of Agaricales.
Sample:
SAMN10245926 • SRS3991098 • All experiments • All runs
Library:
Name: TRC661.87_GD7
Instrument: PacBio RS II
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 163,482 spots, 3.6G bases, 11.6Gb
Run# of Spots# of BasesSizePublished
SRR8127674163,4823.6G11.6Gb2020-05-08

ID:
6677045

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